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  Indian J Med Microbiol
 

Figure 6: Inhibitory effects of SB203580, U0126, SP600125, and JSH23 on the generation of nitric oxide (a), tumor necrosis factor-α (a), interleukin-1 β (b), and interleukin-6 (c) were induced by safrole in RAW264.7 macrophages. The cells incubated with SB203580 at 30 μM, U0126 at 1 μM, SP600125 at 30 μM, and JSH23 at 30 μM for 1 h, and then the cells treated with safrole at 300 μM for 12 h. The nitric oxide generation was measured by Griess reaction. The secretion of tumor necrosis factor-α, interleukin-1 β, and interleukin-6 were detected by by Enzyme-linked immunosorbent assay kits. Results are expressed as means ± standard deviation (n = 3). *P < 0.05 considers significant as compared with control group. P < 0.05 considers significant as compared with safrole group

Figure 6: Inhibitory effects of SB203580, U0126, SP600125, and JSH23 on the generation of nitric oxide (a), tumor necrosis factor-α (a), interleukin-1 β (b), and interleukin-6 (c) were induced by safrole in RAW264.7 macrophages. The cells incubated with SB203580 at 30 μM, U0126 at 1 μM, SP600125 at 30 μM, and JSH23 at 30 μM for 1 h, and then the cells treated with safrole at 300 μM for 12 h. The nitric oxide generation was measured by Griess reaction. The secretion of tumor necrosis factor-α, interleukin-1 β, and interleukin-6 were detected by by Enzyme-linked immunosorbent assay kits. Results are expressed as means ± standard deviation (<i>n</i> = 3). *<i>P</i> < 0.05 considers significant as compared with control group. <i>P</i> < 0.05 considers significant as compared with safrole group